Search results for " Throughput"
showing 10 items of 24 documents
Exploring diversity and biotechnological potential of lactic acid bacteria from tocosh - traditional Peruvian fermented potatoes - by high throughput…
2018
Lactic acid bacteria (LAB) diversity associated with tocosh, Peruvian traditional fermented potatoes, was for the first time investigated by culturing and high throughput sequencing (HTS) approaches. They were applied on three samples i.e. freshly harvested potatoes, one-month and eight-months production. While by culture-dependent approach a few Lactobacillus (Lb) species (Lb. sakei, Lb. casei, Lb. farciminis, Lb. brevis, Lb. fermentum) and Leuconostoc (Ln) mesenteroides were identified, twenty-four OTUs belonging to six LAB genera were considered in tocosh samples by HTS, being Lactobacillus dominant in all three samples. LAB predominated on fresh potatoes, while Clostridium, Zymophilus a…
Human-Driven Microbiological Contamination of Benthic and Hyporheic Sediments of an Intermittent Peri-Urban River Assessed from MST and 16S rRNA Gene…
2017
International audience; Rivers are often challenged by fecal contaminations. The barrier effect of sediments against fecal bacteria was investigated through the use of a microbial source tracking (MST) toolbox, and by Next Generation Sequencing (NGS) of V5-V6 16S rRNA gene (rrs) sequences. Non-metric multi-dimensional scaling analysis of V5-V6 16S rRNA gene sequences differentiated bacteriomes according to their compartment of origin i.e., surface water against benthic and hyporheic sediments. Classification of these reads showed the most prevalent operating taxonomic units (OTU) to be allocated to Flavobacterium and Aquabacterium. Relative numbers of Gaiella, Haliangium, and Thermoleophilu…
Modeling a Novel Variant of Glycogenosis IXa Using a Clonal Inducible Reprogramming System to Generate "Diseased" Hepatocytes for Accurate Diagnosis.
2022
The diagnosis of inherited metabolic disorders is a long and tedious process. The matching of clinical data with a genomic variant in a specific metabolic pathway is an essential step, but the link between a genome and the clinical data is normally difficult, primarily for new missense variants or alterations in intron sequences. Notwithstanding, elucidation of the pathogenicity of a specific variant might be critical for an accurate diagnosis. In this study, we described a novel intronic variant c.2597 + 5G > T in the donor splice sequence of the PHKA2 gene. To investigate PHKA2 mRNA splicing, as well as the functional consequences on glycogen metabolism, we generated hepatocyte-like ce…
Fairness aware rate adaptation and proportional scheduling for IEE 802.11 wlans using FSE
2015
With its rapid development in the wireless markets, IEEE 802.11 WLAN is experiencing a huge popularity. However, due to the limitation of frequency bandwidth of WLANs, it is essential that the available radio resource should be fully utilized to offer different services to multiple users. In order to maximize system throughput while still guaranteeing the fairness among users, a proportional fairness based algorithm is proposed in this work. Since most of the previous resource allocation algorithms were simply based on the channel conditions without taking into account user’s demand, in this paper, we introduce the theory of fuzzy synthetic evaluation (FSE) which also allows us to consider …
Out-of-Band Signaling Scheme for High Speed Wireless LANs
2007
In recent years, the physical layer data rate provided by 802.11 Wireless LANs has dramatically increased thanks to significant advances in the modulation and coding techniques employed. However, previous studies show that the 802.11 MAC operation, namely the distributed coordination function (DCF), represents a limiting factor: the throughput efficiency drops as the channel bit rate increases, and a throughput upper limit does indeed exist when the channel bit rate goes to infinite high. These findings indicate that the performance of the DCF protocol will not be efficiently improved by merely increasing the channel bit rate. This paper shows that the DCF performance may significantly bene…
Insight on bacteria communities in outdoor bronze and marble artefacts in a changing environment
2022
Epilithic bacteria play a fundamental role in the conservation of cultural heritage (CH) materials. On stones, bacterial communities cause both degradation and bioprotection actions. Bronze biocorrosion in non-burial conditions is rarely studied. Only few studies have examined the relationship between bacteria communities and the chemical composi- tion of patinas (surface degradation layers). A better comprehension of bacterial communities growing on our CH is fundamental not only to understand the related decay mechanisms but also to foresee possible shifts in their compo- sition due to climate change. The present study aims at (1) characterizing bacterial communities on bronze andmarble s…
Assessment of fungi proliferation and diversity in cultural heritage: Reactions to UV-C treatment.
2018
International audience; Fungi are present in natural and non-touristic caves due to the presence of organic matter provided mainly by insects or animals such as bats. In show caves, however, tourist infrastructure and the visitors themselves are an important source of organic matter. In addition, photosynthetic biofilms provide a high amount of carbon and nitrogen sources for fungi. This study was conducted to identify the fungal communities present in caves along with the potential use of UV-C treatment against their proliferation. Thus, fungal communities proliferating in biofilms in six French and Swiss show caves were analyzed using high throughput sequencing. The results show 385 speci…
Geosphere-biosphere interactions in bio-activity volcanic lakes: Evidences from Hule and Rìo Cuarto (Costa Rica)
2014
Hule and R ́ıo Cuarto are maar lakes located 11 and 18 km N of Poa ́s volcano along a 27 km long fracture zone, in the Central Volcanic Range of Costa Rica. Both lakes are characterized by a stable thermic and chemical stratification and recently they were affected by fish killing events likely related to the uprising of deep anoxic waters to the surface caused by rollover phenomena. The vertical profiles of temperature, pH, redox potential, chemical and isotopic compositions of water and dissolved gases, as well as prokaryotic diversity estimated by DNA fingerprinting and massive 16S rRNA pyrosequencing along the water column of the two lakes, have highlighted that different bio-geochemica…
Application of Nanogen microarray technology for forensic SNP analysis
2006
Abstract The NanoChip® Molecular Biology Workstation using electronic microarrays is an approach for rapid and high throughput analysis of SNPs. This instrument is fully automated and uses a microchip for electronic addressing of capture probes to specific array sites followed by electronic hybridisation of the single stranded PCR products, and passive hybridisation of fluorescently labelled reporter probes. Discrimination is achieved by applying thermal stringency to denature the mismatched reporters. 48 SNP assays have been designed using the ‘capture down’ assay which applies a thermal ‘touch down’ strategy to obtain the best reporter probe discrimination.
Phenotyping plant root and their interaction with soil micro-organisms
2014
International audience